Denise C. Hocking, Ph.D.
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Denise C. Hocking, Ph.D.
About Me
Faculty Appointments
Professor - Department of Pharmacology and Physiology (SMD)
Professor - Department of Biomedical Engineering (SMD) - Joint
Credentials
Post-doctoral Training & Residency
Postdoctoral Research Fellow; Albany Medical College, Albany, NY.
Topic: "Mechanisms of extracellular matrix fibronectin deposition".
Advisor: Paula J. McKeown-Longo, Ph.D. 1992 - 1996
Education
PhD | Albany Medical College. Physiology. 1992
MS | Albany Medical College. Physiology. 1990
BS | Hartwick College. Medical Technology. 1983
Awards
Proctor and Gamble Career Opportunity Award, American Physiological Society. 1990
Research
The Extracellular Matrix: What is it? The extracellular matrix (ECM) is a complex, three-dimensional network comprised of collagens, glycoproteins, and proteoglycans that provides an adhesive substrate for the organization of cells into tissues. In the adult, dynamic interactions between cells and their surrounding ECM proteins regulate most, if not all, cell and tissue functions. ECM proteins also contribute to the mechanical and permeability properties of the skin, vasculature, lungs, and other organs. Fibronectin is a principal component of the ECM, where it is organized into elongated, branching fibrils. Mechanical forces influence the deposition, organization, and structure of ECM fibronectin fibrils, which in turn, affect cell function, ECM organization and stability, vascular perfusion, blood vessel permeability, and tissue strength. Fibronectin is initially secreted by cells in a soluble, protomeric form. Soluble fibronectins are then polymerized into ECM fibrils by means of a tightly-regulated, cell-dependent mechanism that can be rapidly up- or down-regulated. Fibronectin matrix polymerization is increased in response to tissue injury; altered fibronectin matrix deposition is associated with abnormal tissue repair in a number of chronic inflammatory states, including non-healing wounds, asthma, pulmonary fibrosis, and atherosclerosis. In the body, fibronectin polymerization is a continuous process, with as much as 50% of the fibronectin matrix undergoing turnover every 24 hours. In spite of this remarkable information, the mechanisms of fibronectin matrix polymerization and the function of ECM fibronectin fibrils in vivo is not well understood.
Studies from the Hocking Lab: What have we learned about fibronectin? Research in the Hocking lab has been directed at determining whether and how this three-dimensional fibronectin matrix regulates cell behaviors that are essential to tissue repair. Our studies have firmly established that cells respond differently to soluble versus ECM fibronectin, and that the ECM form is the primary functional form of fibronectin. We have shown that the conversion of fibronectin into the ECM form specifically stimulates cell spreading, cell migration, and collagen matrix contraction – all cellular events critical to tissue regeneration. We have also shown that the assembly of a fibronectin matrix promotes the co-polymerization of collagen I and enhances the tensile strength of model tissue, thus demonstrating that the organization and functional properties of the ECM are also dependent on fibronectin matrix polymerization. We have localized several of the ECM-specific effects of fibronectin to a single, cryptic heparin-binding site in the first type III module of fibronectin (FNIII-1). This conformation-dependent, or "matricryptic" site is not exposed in soluble fibronectin, but becomes unmasked during fibronectin matrix formation or as cells and tissues exert tension on ECM fibronectin fibrils. Our exciting new studies, conducted in collaboration with the laboratory of Dr. Ingrid Sarelius, demonstrate that exposure of this matricryptic site in vivo mechanically couples skeletal muscle contraction with local vasodilation. These were the first studies to demonstrate a role for fibronectin fibrils in adult connective tissue in vivo.
Patents
Ultrasound Technology to Control the Spatial Organization of Cells and Proteins in Engineered Tissues
Issue date: June 27, 2017
Patent #: 9,688,962
Country: United States
Inventors: Diane Dalecki, Kelley Garvin, Denise C Hocking
Chimeric Fibronectin Matrix Mimetics and Uses Thereof
Issue date: July 07, 2015
Patent #: 9,072,706
Country: United States
Inventors: Denise C Hocking, Daniel Roy
Chimeric Fibronectin Matrix Mimetics and Uses Thereof
Issue date: February 21, 2017
Patent #: 9,572,869
Country: United States
Inventors: Denise C Hocking, Daniel Roy
Ultrasound Technology to Control the Spatial Organization of Cells and Proteins in Engineered Tissues
Issue date: May 05, 2020
Patent #: 10,640,750
Country: United States
Inventors: Diane Dalecki, Kelley Garvin, Denise C Hocking
Publications
Journal Articles
Comeau ES, Vander Horst MA, Raeman CH, Child SZ, Hocking DC, Dalecki D
Scientific reports.. 2023 September 2613 (1):16082. Epub 09/26/2023.
Norris EG, Pan XS, Hocking DC
The Journal of biological chemistry.. 2023 March 299 (3):102922. Epub 01/18/2023.
Norris EG, Pan XS, Hocking DC
bioRxiv : the preprint server for biology.. 2022 April 11 Epub 04/11/2022.
Vander Horst MA, Raeman CH, Dalecki D, Hocking DC
Ultrasound in medicine & biology.. 2021 April 47 (4):1054-1066. Epub 01/13/2021.
Norris EG, Dalecki D, Hocking DC
Applied sciences.. 2020 April 210 (8)Epub 04/23/2020.
Fernandes NRJ, Reilly NS, Schrock DC, Hocking DC, Oakes PW, Fowell DJ
Frontiers in immunology.. 2020 11 :1501. Epub 07/24/2020.
Norris EG, Dalecki D, Hocking DC
Recent progress in materials.. 2020 2 (3)Epub 07/21/2020.
Norris EG, Majeski J, Wayson SE, Coleman H, Choe R, Dalecki D, Hocking DC
Materials research express.. 2019 December 6 (12)Epub 11/29/2019.
Farrar CS, Rouin GT, Miller BL, Raeman CH, Mooney NA, Hocking DC
Cells.. 2019 October 308 (11)Epub 10/30/2019.
Hocking DC, Dalecki D, Norris EG
Materials today. Bio.. 2019 June 3 Epub 07/22/2019.
Farrar CS, Hocking DC
The Journal of biological chemistry.. 2018 November 30293 (48):18655-18666. Epub 10/15/2018.
Comeau ES, Hocking DC, Dalecki D
Journal of cell science.. 2017 January 1130 (1):232-242. Epub 10/27/2016.
Hocking DC, Brennan JR, Raeman CH
Advances in wound care.. 2016 November 15 (11):495-506. Epub 1900 01 01.
Okech W, Abberton KM, Kuebel JM, Hocking DC, Sarelius IH
American journal of physiology. Heart and circulatory physiology.. 2016 October 1311 (4):H1063-H1071. Epub 08/12/2016.
Brennan JR, Hocking DC
Acta biomaterialia.. 2016 March 132 :198-209. Epub 12/19/2015.
Dalecki D, Mercado KP, Hocking DC
Annals of biomedical engineering.. 2016 March 44 (3):636-48. Epub 11/18/2015.
Sarelius IH, Titus PA, Maimon N, Okech W, Wilke-Mounts SJ, Brennan JR, Hocking DC
The Journal of physiology.. 2016 February 1594 (3):687-97. Epub 01/15/2016.
Carstensen EL, Parker KJ, Dalecki D, Hocking DC
Ultrasound in medicine & biology.. 2016 January 42 (1):1-15. Epub 10/14/2015.
Hocking DC
Advances in wound care.. 2015 August 14 (8):441-443. Epub 1900 01 01.
Mercado KP, Langdon J, Helguera M, McAleavey SA, Hocking DC, Dalecki D
The Journal of the Acoustical Society of America.. 2015 August 138 (2):EL138-44. Epub 1900 01 01.
Mercado KP, Helguera M, Hocking DC, Dalecki D
Tissue engineering. Part C, Methods.. 2015 July 21 (7):671-82. Epub 03/12/2015.
Dalecki D, Hocking DC
Annals of biomedical engineering.. 2015 March 43 (3):747-61. Epub 10/18/2014.
Mercado KP, Helguera M, Hocking DC, Dalecki D
Annals of biomedical engineering.. 2014 June 42 (6):1292-304. Epub 03/14/2014.
Dalecki D, Hocking DC
The Journal of the Acoustical Society of America.. 2014 April 135 (4):2337. Epub 1900 01 01.
Hocking DC, Raeman CH, Dalecki D
The Journal of the Acoustical Society of America.. 2014 April 135 (4):2370. Epub 1900 01 01.
Mercado KP, Helguera M, Hocking DC, Dalecki D
The Journal of the Acoustical Society of America.. 2014 April 135 (4):2373. Epub 1900 01 01.
Gildner CD, Roy DC, Farrar CS, Hocking DC
Matrix biology : journal of the International Society for Matrix Biology.. 2014 February 34 :33-45. Epub 02/06/2014.
Roy DC, Mooney NA, Raeman CH, Dalecki D, Hocking DC
Tissue engineering. Part A.. 2013 November 19 (21-22):2517-26. Epub 08/12/2013.
Garvin KA, Dalecki D, Yousefhussien M, Helguera M, Hocking DC
The Journal of the Acoustical Society of America.. 2013 August 134 (2):1483-90. Epub 1900 01 01.
Garvin KA, Vanderburgh J, Hocking DC, Dalecki D
The Journal of the Acoustical Society of America.. 2013 August 134 (2):1491-502. Epub 1900 01 01.
Roy DC, Hocking DC
Tissue engineering. Part A.. 2013 February 19 (3-4):558-70. Epub 11/01/2012.
Sevilla CA, Dalecki D, Hocking DC
PloS one.. 2013 8 (10):e77316. Epub 10/08/2013.
Dalecki D, Hocking DC
The Journal of the Acoustical Society of America.. 2012 September 132 (3):1907. Epub 1900 01 01.
Hocking DC, Garvin KA, Dalecki D
The Journal of the Acoustical Society of America.. 2012 September 132 (3):1952. Epub 1900 01 01.
Mercado KP, Helguera M, Hocking DC, Dalecki D
The Journal of the Acoustical Society of America.. 2012 September 132 (3):2066. Epub 1900 01 01.
Garvin KA, Dalecki D, Hocking DC
Ultrasound in medicine & biology.. 2011 November 37 (11):1853-64. Epub 09/15/2011.
Roy DC, Wilke-Mounts SJ, Hocking DC
Biomaterials.. 2011 March 32 (8):2077-87. Epub 12/24/2010.
Lefort CT, Wojciechowski K, Hocking DC
The Journal of biological chemistry.. 2011 January 28286 (4):3149-60. Epub 11/17/2010.
Sevilla CA, Dalecki D, Hocking DC
Tissue engineering. Part A.. 2010 December 16 (12):3805-19. Epub 09/06/2010.
Garvin KA, Hocking DC, Dalecki D
Ultrasound in medicine & biology.. 2010 November 36 (11):1919-32. Epub 09/27/2010.
Garvin KA, Hocking DC, Dalecki D
The Journal of the Acoustical Society of America.. 2010 March 127 (3):1940. Epub 1900 01 01.
Hocking DC, Titus PA, Sumagin R, Sarelius IH
Circulation research.. 2008 February 15102 (3):372-9. Epub 11/21/2007.
Gui L, Wojciechowski K, Gildner CD, Nedelkovska H, Hocking DC
The Journal of biological chemistry.. 2006 November 17281 (46):34816-25. Epub 09/18/2006.
Gildner CD, Lerner AL, Hocking DC
American journal of physiology. Heart and circulatory physiology.. 2004 July 287 (1):H46-53. Epub 03/04/2004.
Wojciechowski K, Chang CH, Hocking DC
Protein expression and purification.. 2004 July 36 (1):131-8. Epub 1900 01 01.
Hocking DC, Chang CH
American journal of physiology. Lung cellular and molecular physiology.. 2003 July 285 (1):L169-79. Epub 03/14/2003.
Hocking DC
Chest.. 2002 December 122 (6 Suppl):275S-278S. Epub 1900 01 01.
Sottile J, Hocking DC
Molecular biology of the cell.. 2002 October 13 (10):3546-59. Epub 1900 01 01.
Hocking DC, Kowalski K
The Journal of cell biology.. 2002 July 8158 (1):175-84. Epub 07/08/2002.
Pereira M, Rybarczyk BJ, Odrljin TM, Hocking DC, Sottile J, Simpson-Haidaris PJ
Journal of cell science.. 2002 February 1115 (Pt 3):609-17. Epub 1900 01 01.
Sottile J, Hocking DC, Langenbach KJ
Journal of cell science.. 2000 December 113 Pt 23 :4287-99. Epub 1900 01 01.
Hocking DC, Sottile J, Langenbach KJ
The Journal of biological chemistry.. 2000 April 7275 (14):10673-82. Epub 1900 01 01.
Hocking DC, Sottile J, Reho T, Fässler R, McKeown-Longo PJ
The Journal of biological chemistry.. 1999 September 17274 (38):27257-64. Epub 1900 01 01.
Sottile J, Hocking DC, Swiatek PJ
Journal of cell science.. 1998 October 111 ( Pt 19) :2933-43. Epub 1900 01 01.
Hocking DC, Sottile J, McKeown-Longo PJ
The Journal of cell biology.. 1998 April 6141 (1):241-53. Epub 1900 01 01.
Hocking DC, Smith RK, McKeown-Longo PJ
The Journal of cell biology.. 1996 April 133 (2):431-44. Epub 1900 01 01.
Hocking DC, Sottile J, McKeown-Longo PJ
The Journal of biological chemistry.. 1994 July 22269 (29):19183-7. Epub 1900 01 01.
Ferro TJ, Hocking DC, Johnson A
The American journal of physiology.. 1993 November 265 (5 Pt 1):L462-71. Epub 1900 01 01.
Johnson A, Hocking DC, Ferro TJ
The American journal of physiology.. 1991 October 261 (4 Pt 2):H996-1004. Epub 1900 01 01.
Hocking DC, Ferro TJ, Johnson A
Journal of applied physiology.. 1991 March 70 (3):1121-8. Epub 1900 01 01.
Hocking DC, Phillips PG, Ferro TJ, Johnson A
Circulation research.. 1990 July 67 (1):68-77. Epub 1900 01 01.
Johnson A, Hocking DC, Ferro TJ
The American journal of physiology.. 1990 January 258 (1 Pt 2):H85-91. Epub 1900 01 01.