Yi-Fen Lee, Ph.D.
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Yi-Fen Lee, Ph.D.
About Me
This is a relatively new and exciting area of research: small vesicles, i...
This is a relatively new and exciting area of research: small vesicles, in size ranging from 30-250 nm, are secreted by almost all types of cells and contain bio-information (such as DNA, RNA and proteins) resembling the cells of origins. They travel distances via circulation, and can be taken up by recipient cells, consequently affecting their behavior. The genetic and molecular contents of EVs can become an attractive resource of biomarkers for prediction of disease and response to therapy.
In my lab, we have isolated EVs from cancer cell lines and urine and blood of patients with bladder cancer at different stages. Via miRNA array, gene array, and proteomics, we are aiming to identify the signature protein profiles that can be used for disease prognosis. Through the CRISPR/Cas9 gene editing system, we have attempted to inhibit cancer growth by targeting EV biogenesis pathways in cancer cells.
We are also studying potential involvement of EV secretion in response to two treatments that often apply in bladder cancer: BCG immunotherapy for superficial bladder cancer, and cisplatin chemotherapy for muscle invasive bladder cancer. Since the EVs are quite small - the size of a virus - we utilize the Nanosight nanoparticle tracking system, which allow us to trace and monitor EVs based on the Brownian motion of each single particle in a real time manner. In collaborations with Â鶹ÊÓƵinvestigators, in addition to our participation in the SWOG S1602 clinical trial, we’ve started to collect and analyze patients’ urine- and blood-borne EV profiles before, during, and after therapy. Our goal is to establish rapid body fluid biopsy assays that can be used as indicators of patients’ responsiveness to therapy. The molecular/genetic contents within EVs from each individual patient could provide an opportunity to develop precision, personalized medicine in the future.
Yi-Fen Lee, Ph.D.
Yi-Fen Lee, Ph.D.
Principal Investigator
Faculty Appointments
Professor - Department of Urology (SMD)
Credentials
Post-doctoral Training & Residency
Post-Doctoral Fellow, Department of Pathology 1997 - 2001
Lab Manager, Department of Pathology 1997 - 2002
Education
PhD | Univ Wisconsin-Madison. Endocrinology/Molecular Oncology. 1997
MS | Tung-Hai University. Biology. 1988
BS | Tung-Hai University. Biology. 1986
Awards
Research Scholar. 2006
Edwin Beer Fellowship Award. 2003 - 2005
Cap CURE Award, Third Prize. 2002
Research
This is a relatively new and exciting area of research: small vesicles, i...
This is a relatively new and exciting area of research: small vesicles, in size ranging from 30-250 nm, are secreted by almost all types of cells and contain bio-information (such as DNA, RNA and proteins) resembling the cells of origins. They travel distances via circulation, and can be taken up by recipient cells, consequently affecting their behavior. The genetic and molecular contents of EVs can become an attractive resource of biomarkers for prediction of disease and response to therapy.
In my lab, we have isolated EVs from cancer cell lines and urine and blood of patients with bladder cancer at different stages. Via miRNA array, gene array, and proteomics, we are aiming to identify the signature protein profiles that can be used for disease prognosis. Through the CRISPR/Cas9 gene editing system, we have attempted to inhibit cancer growth by targeting EV biogenesis pathways in cancer cells.
We are also studying potential involvement of EV secretion in response to two treatments that often apply in bladder cancer: BCG immunotherapy for superficial bladder cancer, and cisplatin chemotherapy for muscle invasive bladder cancer. Since the EVs are quite small - the size of a virus - we utilize the Nanosight nanoparticle tracking system, which allow us to trace and monitor EVs based on the Brownian motion of each single particle in a real time manner. In collaborations with Â鶹ÊÓƵinvestigators, in addition to our participation in the SWOG S1602 clinical trial, we’ve started to collect and analyze patients’ urine- and blood-borne EV profiles before, during, and after therapy. Our goal is to establish rapid body fluid biopsy assays that can be used as indicators of patients’ responsiveness to therapy. The molecular/genetic contents within EVs from each individual patient could provide an opportunity to develop precision, personalized medicine in the future.
Yi-Fen Lee, Ph.D.
Yi-Fen Lee, Ph.D.
Principal Investigator
Publications
Journal Articles
Groves AM, Paris N, Hernady E, Johnston CJ, Aljitawi O, Lee YF, Kerns SL, Marples B
International journal of radiation oncology, biology, physics.. 2023 March 15115 (4):972-982. Epub 11/16/2022.
Molony RD, Wu CH, Lee YF
Scientific reports.. 2023 January 413 (1):142. Epub 01/04/2023.
Ortiz-Bonilla CJ, Uccello TP, Gerber SA, Lord EM, Messing EM, Lee YF
International journal of molecular sciences.. 2022 March 823 (6)Epub 03/08/2022.
Silvers CR, Messing EM, Miyamoto H, Lee YF
British journal of cancer.. 2021 November 125 (10):1399-1407. Epub 09/25/2021.
Chung WM, Molony RD, Lee YF
Stem cell research & therapy.. 2021 October 912 (1):533. Epub 10/09/2021.
Lai GR, Lee YF, Yan SJ, Ting HJ
American journal of physiology. Cell physiology.. 2020 May 1318 (5):C836-C847. Epub 03/11/2020.
Wu CH, Silvers CR, Messing EM, Lee YF
The Journal of biological chemistry.. 2019 March 1294 (9):3207-3218. Epub 12/28/2018.
Joseph JV, Brasacchio R, Fung C, Reeder J, Bylund K, Sahasrabudhe D, Yeh SY, Ghazi A, Fultz P, Rubens D, Wu G, Singer E, Schwarz E, Mohile S, Mohler J, Theodorescu D, Lee YF, Okunieff P, McConkey D, Rashid H, Chang C, Fradet Y, Guru K, Kukreja J, Sufrin G, Lotan Y, Bailey H, Noyes K, Schwartz S, Rideout K, Bratslavsky G, Campbell SC, Derweesh I, Abrahamsson PA, Soloway M, Gomella L, Golijanin D, Svatek R, Frye T, Lerner S, Palapattu G, Wilding G, Droller M, Trump D
Bladder cancer.. 2018 October 34 (Suppl 1):S1-S43. Epub 10/03/2018.
Liu YR, Ortiz-Bonilla CJ, Lee YF
International journal of molecular sciences.. 2018 September 1819 (9)Epub 09/18/2018.
Silvers CR, Miyamoto H, Messing EM, Netto GJ, Lee YF
Oncotarget.. 2017 October 318 (53):91199-91208. Epub 08/08/2017.
Silvers CR, Liu YR, Wu CH, Miyamoto H, Messing EM, Lee YF
Oncotarget.. 2016 April 267 (17):23335-45. Epub 1900 01 01.
Yasmin-Karim S, King MR, Messing EM, Lee YF
Oncotarget.. 2014 December 155 (23):12097-110. Epub 1900 01 01.
Beckham CJ, Olsen J, Yin PN, Wu CH, Ting HJ, Hagen FK, Scosyrev E, Messing EM, Lee YF
The Journal of urology.. 2014 August 192 (2):583-92. Epub 02/14/2014.
Lin SJ, Lee SO, Lee YF, Miyamoto H, Yang DR, Li G, Chang C
Carcinogenesis.. 2014 June 35 (6):1399-406. Epub 02/28/2014.
Hsu JW, Yin PN, Wood R, Messing J, Messing E, Lee YF
Oncotarget.. 2013 December 4 (12):2397-406. Epub 1900 01 01.
Ting HJ, Messing J, Yasmin-Karin S, Lee YF
The Journal of biological chemistry.. 2013 January 4288 (1):1-9. Epub 11/27/2012.
Lin SJ, Ho HC, Lee YF, Liu NC, Liu S, Li G, Shyr CR, Chang C
Reproductive biology and endocrinology : RB&E.. 2012 June 710 :43. Epub 06/07/2012.
Yan SJ, Lee YF, Ting HJ, Liu NC, Liu S, Lin SJ, Yeh SD, Li G, Chang C
Cellular & molecular biology letters.. 2012 June 17 (2):309-22. Epub 03/07/2012.
Ting HJ, Yasmin-Karim S, Yan SJ, Hsu JW, Lin TH, Zeng W, Messing J, Sheu TJ, Bao BY, Li W, Messing E, Lee YF
Cancer research.. 2012 February 1572 (4):958-68. Epub 12/29/2011.
Liu S, Yan SJ, Lee YF, Liu NC, Ting HJ, Li G, Wu Q, Chen LM, Chang C
The Journal of biological chemistry.. 2011 November 4286 (44):38103-38108. Epub 09/14/2011.
Collins LL, Lee YF, Ting HJ, Lin WJ, Liu NC, Meshul CK, Uno H, Bao BY, Chen YT, Chang C
Reproductive biology and endocrinology : RB&E.. 2011 October 139 :138. Epub 10/13/2011.
Liu S, Lee YF, Chou S, Uno H, Li G, Brookes P, Massett MP, Wu Q, Chen LM, Chang C
Molecular endocrinology.. 2011 August 25 (8):1301-10. Epub 05/26/2011.
Lee YF, Liu S, Liu NC, Wang RS, Chen LM, Lin WJ, Ting HJ, Ho HC, Li G, Puzas EJ, Wu Q, Chang C
American journal of physiology. Endocrinology and metabolism.. 2011 July 301 (1):E91-8. Epub 04/26/2011.
Xie S, Ni J, Lee YF, Liu S, Li G, Shyr CR, Chang C
The Journal of biological chemistry.. 2011 June 17286 (24):21129-36. Epub 04/22/2011.
Kim E, Liu NC, Yu IC, Lin HY, Lee YF, Sparks JD, Chen LM, Chang C
Diabetes.. 2011 May 60 (5):1493-503. Epub 04/08/2011.
Hsu JW, Yasmin-Karim S, King MR, Wojciechowski JC, Mickelsen D, Blair ML, Ting HJ, Ma WL, Lee YF
The American journal of pathology.. 2011 February 178 (2):872-80. Epub 1900 01 01.
Bao BY, Ting HJ, Hsu JW, Yasmin-Karim S, Messing E, Lee YF
The Journal of steroid biochemistry and molecular biology.. 2010 May 120 (1):11-21. Epub 03/04/2010.
Liu NC, Lin WJ, Yu IC, Lin HY, Liu S, Lee YF, Chang C
Endocrine.. 2009 October 36 (2):211-7. Epub 07/18/2009.
Xie S, Lee YF, Kim E, Chen LM, Ni J, Fang LY, Liu S, Lin SJ, Abe JI, Berk B, Ho FM, Chang C
Proceedings of the National Academy of Sciences of the United States of America.. 2009 August 11106 (32):13353-8. Epub 07/28/2009.
Cai Y, Lee YF, Li G, Liu S, Bao BY, Huang J, Hsu CL, Chang C
International journal of cancer.. 2008 July 1123 (1):195-201. Epub 1900 01 01.
Li G, Lee YF, Liu S, Cai Y, Xie S, Liu NC, Bao BY, Chen Z, Chang C
Endocrinology.. 2008 July 149 (7):3490-9. Epub 04/03/2008.
Bao BY, Ting HJ, Hsu JW, Lee YF
International journal of cancer.. 2008 June 15122 (12):2699-706. Epub 1900 01 01.
Chen LM, Wang RS, Lee YF, Liu NC, Chang YJ, Wu CC, Xie S, Hung YC, Chang C
Molecular endocrinology.. 2008 April 22 (4):858-67. Epub 01/03/2008.
Liu NC, Lin WJ, Kim E, Collins LL, Lin HY, Yu IC, Sparks JD, Chen LM, Lee YF, Chang C
Diabetes.. 2007 December 56 (12):2901-9. Epub 09/07/2007.
Ting HJ, Bao BY, Reeder JE, Messing EM, Lee YF
Molecular cancer research : MCR.. 2007 September 5 (9):967-80. Epub 1900 01 01.
Zhang Y, Chen YT, Xie S, Wang L, Lee YF, Chang SS, Chang C
Molecular endocrinology.. 2007 April 21 (4):908-20. Epub 01/16/2007.
Ting HJ, Hsu J, Bao BY, Lee YF
Cancer letters.. 2007 March 8247 (1):122-9. Epub 04/27/2006.
Chen TC, Persons KS, Zheng S, Mathieu J, Holick MF, Lee YF, Bao B, Arai MA, Kittaka A
The Journal of steroid biochemistry and molecular biology.. 2007 March 103 (3-5):717-20. Epub 01/04/2007.
Evaluation of C-2-substituted 19-nor-1alpha,25-dihydroxyvitamin D(3) analogs as therapeutic agents for prostate cancer
Chen,T. C.; Persons,K. S.; Zheng,S.; Mathieu,J.; Holick,M. F.; Lee,Y. F.; Bao,B.; Arai,M. A.; Kittaka,A.;.
J.Steroid Biochem.Mol.Biol. 2007; .
Loss of Testicular Orphan Receptor 4 Impairs Normal Myelination in Mouse Forebrain
Zhang,Y.; Chen,Y. T.; Xie,S.; Wang,L.; Lee,Y. F.; Chang,S. S.; Chang,C.;.
Mol.Endocrinol. 2007; .
Wang CP, Lee YF, Chang C, Lee HJ
Biochemical and biophysical research communications.. 2006 December 8351 (1):204-8. Epub 10/12/2006.
Bao BY, Yao J, Lee YF
Carcinogenesis.. 2006 September 27 (9):1883-93. Epub 04/19/2006.
Bao BY, Yeh SD, Lee YF
Carcinogenesis.. 2006 January 27 (1):32-42. Epub 06/29/2005.
Chuang KH, Lee YF, Lin WJ, Chu CY, Altuwaijri S, Wan YJ, Chang C
Molecular endocrinology.. 2005 May 19 (5):1200-12. Epub 01/13/2005.
Ting HJ, Bao BY, Hsu CL, Lee YF
Endocrine.. 2005 February 26 (1):1-9. Epub 1900 01 01.
Lee YF, Bao BY, Chang C
Biochemical and biophysical research communications.. 2004 October 22323 (3):876-83. Epub 1900 01 01.
Collins LL, Lee YF, Heinlein CA, Liu NC, Chen YT, Shyr CR, Meshul CK, Uno H, Platt KA, Chang C
Proceedings of the National Academy of Sciences of the United States of America.. 2004 October 19101 (42):15058-63. Epub 10/11/2004.
Lee YF, John M, Falconer A, Edwards S, Clark J, Flohr P, Roe T, Wang R, Shipley J, Grimer RJ, Mangham DC, Thomas JM, Fisher C, Judson I, Cooper CS
Cancer research.. 2004 October 1564 (20):7201-4. Epub 1900 01 01.
Chen HH, Lee YF, Chan MH, Lo PS
Annals of the New York Academy of Sciences.. 2004 October 1025 :552-5. Epub 1900 01 01.
Mu X, Lee YF, Liu NC, Chen YT, Kim E, Shyr CR, Chang C
Molecular and cellular biology.. 2004 July 24 (13):5887-99. Epub 1900 01 01.
Bao BY, Hu YC, Ting HJ, Lee YF
Oncogene.. 2004 April 2223 (19):3350-60. Epub 1900 01 01.
Kim E, Xie S, Yeh SD, Lee YF, Collins LL, Hu YC, Shyr CR, Mu XM, Liu NC, Chen YT, Wang PH, Chang C
The Journal of biological chemistry.. 2003 November 21278 (47):46919-26. Epub 09/03/2003.
Lin WJ, Li J, Lee YF, Yeh SD, Altuwaijri S, Ou JH, Chang C
The Journal of biological chemistry.. 2003 March 14278 (11):9353-60. Epub 01/08/2003.
Inui S, Lee YF, Chang E, Shyr CR, Chang C
Journal of dermatological science.. 2003 February 31 (1):65-71. Epub 1900 01 01.
Lee YF, Lin WJ, Huang J, Messing EM, Chan FL, Wilding G, Chang C
Cancer research.. 2002 November 162 (21):6039-44. Epub 1900 01 01.
Lee YF, Lee HJ, Chang C
The Journal of steroid biochemistry and molecular biology.. 2002 August 81 (4-5):291-308. Epub 1900 01 01.
Lee HJ, Lee YF, Chang C
Biochemical and biophysical research communications.. 2001 August 3285 (5):1361-8. Epub 1900 01 01.
Lee YF, Shyr CR, Thin TH, Lin WJ, Chang C
Proceedings of the National Academy of Sciences of the United States of America.. 1999 December 2196 (26):14724-9. Epub 1900 01 01.
Lee YF, Young WJ, Lin WJ, Shyr CR, Chang C
The Journal of biological chemistry.. 1999 June 4274 (23):16198-205. Epub 1900 01 01.
Inui S, Lee YF, Haake AR, Goldsmith LA, Chang C
The Journal of investigative dermatology.. 1999 April 112 (4):426-31. Epub 1900 01 01.
Lee HJ, Lee YF, Chang C
Molecular and cellular biochemistry.. 1999 April 194 (1-2):199-207. Epub 1900 01 01.
Young WJ, Lee YF, Smith SM, Chang C
The Journal of biological chemistry.. 1998 August 14273 (33):20877-85. Epub 1900 01 01.
Lee YF, Young WJ, Burbach JP, Chang C
The Journal of biological chemistry.. 1998 May 29273 (22):13437-43. Epub 1900 01 01.
Chang C, Lee HJ, Lee YF
Biochemical and biophysical research communications.. 1997 June 9235 (1):205-11. Epub 1900 01 01.
Lee YF, Pan HJ, Burbach JP, Morkin E, Chang C
The Journal of biological chemistry.. 1997 May 2272 (18):12215-20. Epub 1900 01 01.