URMC / Research / Center for Advanced Light Microscopy and Nanoscopy (CALMN) / Services / Instruments / Zeiss Palm MicroBeam Laser Microdissection Zeiss Palm MicroBeam Laser Capture Microdissection Capabilities: Selectively capture cells or tissue segments from larger cultures or tissue sections for further analysis Epi-fluorescent Microscopy Color Histology/Microscopy Summary of Instrument Features: Microscope: Upright Zeiss Axio Observer Z1 Software: Zeiss AxioVision Objectives: Standard: 63x/1.4 - Oil – Plan Apochromat 60x/0.75 - Air – Plan NeoFluar 40x/0.6 - Air – Plan NeoFluar 20x/0.4 - Air – Plan NeoFluar 10x/0.05 - Air – Fluar Light source: Excitation: Lumen Dynamics X-Cite Series 120Q Lamp Capture: Pulsed UV Laser Cameras: Zeiss CC1 Color Camera ​​Zeiss mRM BW Camera Stage: Motorized stage 3-Slide sample holder 2-Cap holder for sample collection Protocols: RNA extraction from FFPE sections (Zeiss) PALM protocols-DNA handling (Zeiss) RNA extraction from frozen sections (Zeiss) LCM protocols-RNA handling (Zeiss) Laser Microdissection: A key technology for Microproteomics (Zeiss) Tissue scrape protocol for verifying RNA Quality (Arcturus)