URMC / Labs / Dirksen Lab / Techniques
Techniques
We employ several techniques in our laboratory including the following:
Electrophysiological Recording
- Whole-cell voltage and current patch-clamp technique
- Single channel patch-clamp recording
- Perforated patch-clamp recording
- Artificial lipid bilayer single channel recording
Molecular, biochemical, and cell biological techniques:
- Molecular cloning
- Western blotting
- Immunoprecipitation
- Immunostaining
- RT-PCR
- Transgenic gene expression
Optical/Imaging techniques
- Bi-molecular fluorescence complementation
- Fluorescence energy resonance transfer
- Confocal imaging
- Electron microscopy
Physiological assays
- In vitro muscle contraction
- In vivo muscle performance assays